Centrifuges are crucial in clinical research for the precise separation of blood components and biological molecules. They are used to separate red and white blood cells, platelets, and other molecules based on their density and size. They are also used to isolate proteins and other molecules from a blood sample. The centrifuge process can separate components with different densities and sizes by varying the speed of rotation.
Obtaining Serum and Plasma Through Centrifugation
Assays require serum or plasma, which can be obtained through centrifugation. Serum is obtained by clotting a whole blood sample at room temperature, then being removed by a centrifuge. Plasma, on the other hand, is obtained from whole blood containing serum and clotting factors. Anticoagulant tubes are used to collect the sample, and blood cells are separated from the plasma supernatant. This precise separation provides essential information for medical research, helping researchers understand medical conditions and develop treatments.
Applications and Efficiency
Different types of centrifuges cater to specific applications in clinical research, enhancing the accuracy and efficiency of various diagnostic and therapeutic processes. They play a pivotal role in isolating proteins and other molecules from blood samples, which is crucial for assays and developing treatments.
Types of Clinical Centrifuges
- Floor Standing Centrifuges: These centrifuges are versatile and ideal for routine laboratory tasks such as sample preparation and frequently used in blood banks. The Hettich ROTO SILENTA 630 RS is highly efficient for centrifuging blood bags, handling up to 12 litres or 12 blood bags per run, with four and six-place rotor options for optimal fit. It can also accommodate six vessels of up to 2,000 ml each, with a temperature range of -20°C to +40°C, making it suitable for industrial or R&D purposes.
- Refrigerated Centrifuges: Refrigerated centrifuges maintain low temperatures during centrifugation, crucial for temperature-sensitive samples. The Hettich UNIVERSAL 320R is a compact, versatile centrifuge capable of performing various tasks, including cytology, without needing a dedicated cytocentrifuge. It features Hettich’s 12-place Rotor, capable of spinning up to 6 ml single cytology chambers and includes an autoclavable bio-containment lid.
- High-Speed Centrifuges: High-speed centrifuges are designed for rapid separations, essential for tasks like DNA extraction and protein purification. The Hettich ROTINA 380 universal benchtop centrifuge can process up to 4 samples of 290 ml at a max. RCF of 4,863, with angular rotors available for higher speeds up to 16,504 RCF for 50 ml tubes. This user-friendly centrifuge features a lid locking system, emergency release, anti-drop safety lid, automatic rotor recognition, and imbalance shutdown, with TUV-certified accessories for handling toxic or infectious materials.
In conclusion centrifuges are vital to clinical research, providing the means to separate and analyse blood components. Hettich, with their longstanding reputation for durability and innovation, along with over 120 years of expertise, exemplifies utility through their durable and efficient centrifuges. They play a significant role in advancing medical research and improving healthcare outcomes.